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KMID : 1134820100390081132
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Journal of the Korean Society of Food Science and Nutrition
2010 Volume.39 No. 8 p.1132 ~ p.1136
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Biological Activity of Phenol Compound from a Cactus Cheonnyuncho (Opuntia humifusa) in Korea
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Lee Kyung-Seok
Lee Ki-Young
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Abstract
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After Cheonnyuncho (Opuntia humifusa) was extracted by 70% ethanol and partitioned with hexane, chloroform, ethyl acetate, butanol, and water in order, ethyl acetate fraction with excellent antioxidant activities was acquired. Ethyl acetate fraction was conducted by TLC, and the third spot of 10 spots was selected due to its best antioxidant activities. When the third fraction further isolated on silica gel chromatography, the fourth fraction of 10 fractions had the best antioxidant activities and purified by HPLC. The molecular weight on EI-MS, and 1H- and 13C-NMR spectrum showed that the purified compound was taxifolin. In addition, antioxidant activities were analyzed, and the purified compound from Cheonnyuncho was found to be effective as much as α-tocopherol, BHA. According to the analysis on antibacterial activities, the purified compound also showed more activity than benzoic acid against all pathogenic bacteria.
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KEYWORD
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cactus Cheonnyuncho(Opuntia humifusa), antioxidative activity, antimicrobial activity, taxifolin
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